《植物生理学报》 2012, 48(4): 393-396

蛇足石杉原叶体的培养及孢子体的诱导

包日双, 尹培培, 郭斌*, 尉亚辉*

西部资源生物与现代生物技术省部共建教育部重点实验室, 陕西省生物技术重点实验室, 西北大学生命科学学院, 西安710069

通信作者：郭斌；E-mail: weiyahui@nwu.edu.cn, guobin@nwu.edu.cn；Tel: 029-88303484

摘　要：

以蛇足石杉孢子囊为材料培养得到蛇足石杉原叶体, 进一步对原叶体进行增殖培养并诱导产生蛇足石杉孢子体。结果表明, 适合蛇足石杉原叶体增殖的培养基是无激素的MS培养基, 在该培养基上培养60 d, 原叶体的增殖倍数达到131.1;较高浓度的外源激素对原叶体的增殖有抑制作用, 而低浓度的外源激素对原叶体的增殖影响不大; 原叶体在无激素培养基上培养90 d, 孢子体的诱导频率为4.0%; 通过HPLC检测蛇足石杉原叶体中石杉碱甲的含量为0.0059%, 是野生孢子体中含量的0.25倍

关键词：蛇足石杉; 原叶体; 孢子体; 石杉碱甲

收稿：2012-01-09   修定：2012-02-03

资助：国家自然科学基金项目(31000144)和陕西省教育厅科研计划项目(09JK746)。

Prothallium Culture and Sporophyte Induction of Huperzia serrata (Thunb.) Trev.

BAO Ri-Shuang, YIN Pei-Pei, GUO Bin*, WEI Ya-Hui*

Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, Shaanxi Provincial Key Laboratory of Biotechnology, College of Life Science, Northwest University, Xi’an 710069, China

Corresponding author: GUO Bin; E-mail: weiyahui@nwu.edu.cn, guobin@nwu.edu.cn; Tel: 029-88303484

Abstract:

Huperzia serrata prothallium was obtained from sporangium by in vitro culture and prothallium proliferation and sporophyte induction of H.serrata were studied. The results showed that MS medium without any hormone was optimal medium for prothallium proliferation and the prothallium quantity reached 131.1-fold of inoculation after 60 days. High concentration of exogenous hormones had inhibitory effect on prothallium proliferation, while low concentration of exogenous hormones didn’t have obvious effect on prothallium proliferation. The induction frequency of sporophyte was 4.0% after 90 days on MS medium without any hormone. HPLC analysis results showed that the content of huperzine A in the prothallium of H.serrata was 0.0059%, which was a quarter of the content in wild plants.

Key words: Huperzia serrata; prothallium; sporophyte; huperzine A